Nasopharyngeal swabs packaging instructions.

I: With . Nasopharyngeal Sampling Swab 50 sticks Instructions Fresh Tissue Samples

1. Estimate the volume of this product needed to completely submerge the sample (10mL for 1g of tissue).

2. Mark the collection tube and add the estimated amount of product required.

3. Cut the sample into pieces less than 0.5 cm thick as fast as possible.

Note: Small organ samples such as mouse liver, kidney and spleen and plant samples without wax protection layer can be stored in this product without shearing, while plant samples with wax protection layer need to have the wax skin broken first.

4. Completely submerge the tissue fragments in the collection tube of this product.

5. Store the collection tube at an appropriate temperature for no longer than the zui duration of storage at that temperature. For storage at -20°C or -80°C, leave the sample at 4°C overnight before transferring to the zui temperature. Note: Before transferring the sample to -20°C or -80°C, the protective solution needs to be discarded. Common storage temperatures are related to their zui duration as follows.

6. Remove the sample from storage (samples stored at -20°C or -80°C need to be melted at room temperature) and remove the tissue fragments from the protective solution using sterilized forceps.

Immediately start RNA extraction or other processing (e.g., splitting the sample into smaller pieces for re-preservation, etc.). Note: Samples can be repeatedly freeze-thawed up to twenty times without affecting the quality of the RNA.

Notes

1、Nasopharyngeal swabs are not recommended for general bacterial culture, except for specific bacteria such as Bordetella pertussis and Neisseria meningitidis.

2、If B. pertussis infection is suspected, the laboratory should be notified in advance and special transfer medium (Regan-Lowe) should be prepared. Inoculation medium can be provided when conditions permit, straight

3、Transfer to the laboratory after receiving bedside inoculation.

4、Nasopharyngeal swabs cannot be used to test for the pathogenic bacteria of sinusitis.